Publications
25
MIBiG Database
Zdouc, M.M.;* Blin, K.;* Louwen, N.L.L.; Navarro, J.; Loureiro, C.; [142 authors]; Kalkreuter, E.; [136 authors]; van der Hooft, J.J.J.; Linington, R.G.; Weber, T.; Medema, M.H. MIBiG 4.0: advancing biosynthetic gene cluster curation through global collaboration. Nucleic Acids Res. 2024, gkae1115. Link
24
PKS Domain Structure
Steele, A.D.; Meng, S.; Li, G.; Kalkreuter, E.; Chang, C.; Shen, B. Structural insights into the mechanism of a polyketide synthase thiocysteine lyase domain. J. Am. Chem. Soc. 2024, 146, 32605-32617. Link
23
Unified Enediyne Biosynthesis
Gui, C.;* Kalkreuter, E.;* Lauterbach, L.; Yang, D.; Shen, B. Enediyne biosynthesis unified by an iodinated linear tetrayne intermediate. Nat. Chem. Biol. 2024, 20, 1210-1219. Link
22
Natural Products Discovery Center
Kalkreuter, E.;* Kautsar, S.A.;* Yang, D.;* Teijaro, C.N.;* Bader, C.D.;* Fluegel, L.L.;† Davis, C.M.;† Simpson, J.R.;† Steele, A.D.; Gui, C.; Meng, S.; Li, G.; Viehrig, K.; Ye, F.; Su, P.; Kiefer, A.F.; Lauterbach, L.; Nichols, A.; Cepeda, A.J.; Yan, W.; Fan, B.; Jiang, Y.; Adhikari, A.; Zheng, C.-J.; Shen, B. The Natural Products Discovery Center: release of the first 8490 sequenced strains for exploring Actinobacteria biosynthetic diversity. bioRxiv 2023, 2023.12.14.571759. Link
21
PKS ATs and TEs
Steele, A.D.; Jiang, H.; Pan, G.; Lim, S.-K.; Kalkreuter, E.; Kwong, T.; Ju, J.; Rajski, S.; Shen, B. Discrete acyltransferases and thioesterases in iso-migrastatin and lactimidomycin biosynthesis. Biochemistry, 2024, 63, 563-575. Link
20
Microbial Platform Engineering
Fluegel, L.L.;* Deng, M.;* Su, P.;* Kalkreuter, E.; Yang, D.; Rudolf, J.D.; Dong, L.-B.; Shen, B. Development of platensimycin, platencin, and platensilin overproducers by biosynthetic pathway engineering and fermentation medium optimization. J. Ind. Microbiol. Biotechnol., 2024, 51, kuae003. Link
19
Cofactorless Oxygenases
Gui, C.;* Kalkreuter, E.;* Liu, Y.-C.; Li, G.; Steele, A.D.; Yang, D.; Chang, C.; Shen, B. Cofactorless oxygenases guide anthraquinone-fused enediyne biosynthesis. Nat. Chem. Biol., 2024, 20, 243-250. Link
18
Enediyne Origins
Bhardwaj, M.; Cui, Z.; Hankore, F.D.; Moonschi, F.H.; Esfahani, H.S.; Kalkreuter, E.; Gui, C.; Yang, D.; Phillips Jr., G.N.; Thorson, J.S.; Shen, B.; Van Lanen, S.G. A discrete intermediate for the biosynthesis of both the enediyne core and the anthraquinone moiety of enediyne natural products. Proc. Natl. Acad. Sci. USA, 2023, 120, e2220468120. Link
17
MIBiG Database
Terlouw, B.R.; Blin, K.; Navarro-Muñoz, J.C.; Avalon, N.E.; Chevrette, M.G.; Egbert, S.; Lee, S.; Meijer, D.; Recchia, M.J.J.; Reitz, Z.L.; van Santen, J.A.; Selem-Mojica, N.; Tørring, T.; Zaroubi, L.; [25 authors]; Kalkreuter, E.; [38 authors]; Collemare, J.; Linington, R.G.; Weber, T.; Medema, M.H. MIBiG 3.0: a community-driven effort to annotate experimentally validated biosynthetic gene clusters. Nucleic Acids Res., 2022, 51, D603-D610. Link
16
Enediyne-Anthraquinone Fusion
Gui, C.*; Kalkreuter, E.*; Liu, Y.-C.; Adhikari, A.; Teijaro, C.N.; Yang, D.; Chang, C.; Shen, B. Intramolecular C-C bond formation links anthraquinone and enediyne scaffolds in tiancimycin biosynthesis. J. Am. Chem. Soc., 2022, 144, 20452-20462. Link
15
Enzyme Mechanisms
Kalkreuter, E.; Shen, B. MIO-containing aminomutases for α- to β-amino acids. Trends Chem., 2022, 4, 91-92. Mechanism of the Month. Link
14
PKS SH Domain
Meng, S.; Steele, A.D.; Yan, W.; Pan, G.; Kalkreuter, E.; Liu, Y.-C.; Xu, Z.; Shen, B. Thiocysteine lyases as polyketide synthase domains installing hydropersulfide into natural products and a hydropersulfide methyltransferase. Nat. Commun., 2021, 12, 1-11. Link
13
MD-Guided PKS Engineering
Kalkreuter, E.; Bingham, K.S.; Keeler, A.M.; Lowell, A.N.; Schmidt, J.J.; Sherman, D.H.; Williams, G.J. Computationally-guided exchange of substrate selectivity motifs in a modular polyketide synthase acyltransferase. Nat. Commun., 2021, 12, 2193. Link
12
Discovery of Thioangucyclines
Cao, M.; Zheng, C.-J.; Yang, D.; Kalkreuter, E.; Adhikari, A.; Liu, Y.-C.; Rateb, M.E.; Shen, B. Cryptic sulfur incorporation in thioangucycline biosynthesis. Angew. Chem. Int. Ed., 2021, 133, 7216-7223. Link
11
Thioacid Arylamine N-acyltransferase
Zheng, C.-J.*; Kalkreuter, E.*; Fan, B.-Y.; Liu, Y.-C.; Dong, L.-B.; Shen, B. PtmC catalyzes the final step of thioplatensimycin, thioplatencin, and thioplatensilin biosynthesis and expands the scope of arylamine N-acetyltransferases. ACS Chem. Biol., 2020, 16, 96-105. Link
10
Genome-Guided Natural Product Discovery
Kalkreuter, E.; Pan, G.; Cepeda, A.J.; Shen, B. Targeting bacterial genomes for natural product discovery. Trends Pharmacol. Sci., 2020, 41, 13-26. Feature Review. Link
09
Hybrid NRPS-PKSs
Steele, A.D.; Kalkreuter, E.; Pan, G.; Meng, S.; Shen, B. Hybrid peptide-polyketide natural product biosynthesis. In Comprehensive Natural Products III (Third Edition); Liu, H.-W.; Begley, T.P., Eds.; Elsevier: Oxford, 2020; Volume 2, Chapter 13, p 284-335. Link
08
Diterpenoid Monooxygenase
Dong, L.-B.; Liu, Y.-C.; Cepeda, A.J.; Kalkreuter, E.; Deng, M.-R.; Rudolf, J.D.; Chang, C.; Joachimiak, A.J.; Phillips Jr, G.N.; Shen, B. Characterization and crystal structure of a nonheme diiron monooxygenase involved in platensimycin and platencin biosynthesis. J. Am. Chem. Soc., 2019, 141, 12406-12412. Link
07
Malonyl-CoA Biosensors
Kalkreuter, E.; Keeler, A.M.; Malico, A.A.; Bingham, K.S.; Gayen, A.K.; Williams, G.J. Development of a genetically encoded biosensor for detection of polyketide synthase extender units in Escherichia coli. ACS Synth. Biol., 2019, 8, 1391-1400. Link
06
Three-Enzyme Diterpenoid Oxidation
Dong, L.-B.; Zhang, X.; Rudolf, J.D.; Deng, M.-R.; Kalkreuter, E.; Cepeda, A.J.; Renata, H.; Shen, B. Cryptic and stereospecific hydroxylation, oxidation, and reduction in platensimycin and platencin biosynthesis. J. Am. Chem. Soc., 2019, 141, 4043-4050. Link
05
Bimodular PKS Engineering
Kalkreuter, E.; CroweTipton, J.M.; Lowell, A.N.; Sherman, D.H.; Williams, G.J. Engineering the substrate specificity of a modular polyketide synthase for installation of consecutive non-natural extender units. J. Am. Chem. Soc., 2019, 141, 1961-1969. Link
04
Precursor-Directed Biosynthesis
Kalkreuter, E.; Carpenter, S.M.; Williams, G.J. Precursor-directed biosynthesis and semi-synthesis. In Chemical and Biological Synthesis; Westwood, N.J.; Nelson, A., Eds.; The Royal Society of Chemistry: London, 2018; Chapter 11, p 275-312. Link
03
NRPS and PKS Engineering
Kalkreuter, E.; Williams, G.J. Engineering enzymatic assembly lines for the production of new antimicrobials. Curr. Op. Microbiol., 2018, 45, 140-148. Link
02
Nitric Oxide in Prostate Cancer
Sun, Q.; Kalkreuter, E.; Sun, C.Q.; Arnold, R.S.; Petros, J.A. Mitochondrial DNA mutations enhance proliferation via modulation of cellular nitric oxide, an effect amenable to pharmacologic manipulation. J. Urol., 2013, 189, e88-e89. Link
01
Stem Cell Patents
Shyntum, Y.; Kalkreuter, E. Stem cell patents—reexamination/litigation—the last 5 years. Tissue. Eng. Part B Rev., 2009, 15, 87-90. Link